N-Terminal Finger Stabilizes the S1 Pocket for the Reversible Feline Drug GC376 in the SARS-CoV-2 Mpro Dimer.
Arutyunova E, Khan MB, Fischer C, Lu J, Lamer T, Vuong W, van Belkum MJ, McKay RT, Tyrrell DL, Vederas JC, Young HS, Lemieux MJ.
J Mol Biol. 2021 Apr 22;433(13):167003. doi: 10.1016/j.jmb.2021.167003. Online ahead of print.

Insights into the catalytic properties of the mitochondrial rhomboid protease PARL.
Lysyk L, Brassard R, Arutyunova E, Siebert V, Jiang Z, Takyi E, Morrison M, Young HS, Lemberg MK, O'Donoghue AJ, Lemieux MJ.
J Biol Chem. 2021 Feb 5;296:100383. doi: 10.1016/j.jbc.2021.100383. Online ahead of print.

Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy.
Hernando MD, Primeau JO, Young HS.
Methods Mol Biol. 2021;2302:179-199. doi: 10.1007/978-1-0716-1394-8_10.

Author Correction: Feline coronavirus drug inhibits the main protease of SARS-CoV-2 and blocks virus replication.
Vuong W, Khan MB, Fischer C, Arutyunova E, Lamer T, Shields J, Saffran HA, McKay RT, van Belkum MJ, Joyce MA, Young HS, Tyrrell DL, Vederas JC, Lemieux MJ.
Nat Commun. 2020 Oct 20;11(1):5409. doi: 10.1038/s41467-020-19339-y.

Feline coronavirus drug inhibits the main protease of SARS-CoV-2 and blocks virus replication.
Vuong W, Khan MB, Fischer C, Arutyunova E, Lamer T, Shields J, Saffran HA, McKay RT, van Belkum MJ, Joyce MA, Young HS, Tyrrell DL, Vederas JC, Lemieux MJ.
Nat Commun. 2020 Aug 27;11(1):4282. doi: 10.1038/s41467-020-18096-2.

Protein docking and steered molecular dynamics suggest alternative phospholamban-binding sites on the SERCA calcium transporter.
Alford RF, Smolin N, Young HS, Gray JJ, Robia SL.
J Biol Chem. 2020 Aug 7;295(32):11262-11274. doi: 10.1074/jbc.RA120.012948. Epub 2020 Jun 17.

Myocardial MMP-2 contributes to SERCA2a proteolysis during cardiac ischaemia-reperfusion injury.
Roczkowsky A, Chan BYH, Lee TYT, Mahmud Z, Hartley B, Julien O, Armanious G, Young HS, Schulz R.
Cardiovasc Res. 2020 Apr 1;116(5):1021-1031. doi: 10.1093/cvr/cvz207.

Interaction of a Sarcolipin Pentamer and Monomer with the Sarcoplasmic Reticulum Calcium Pump, SERCA.
Glaves JP, Primeau JO, Gorski PA, Espinoza-Fonseca LM, Lemieux MJ, Young HS.
Biophys J. 2020 Jan 21;118(2):518-531. doi: 10.1016/j.bpj.2019.11.3385. Epub 2019 Nov 28.

Regulating the regulator: intramembrane proteolysis of vesicular trafficking proteins and the SERCA regulator phospholamban.
Young HS, Lemieux MJ.
EMBO Rep. 2019 Mar;20(3):e47792. doi: 10.15252/embr.201947792. Epub 2019 Feb 25.

The Phospholamban Pentamer Alters Function of the Sarcoplasmic Reticulum Calcium Pump SERCA.
Glaves JP, Primeau JO, Espinoza-Fonseca LM, Lemieux MJ, Young HS.
Biophys J. 2019 Feb 19;116(4):633-647. doi: 10.1016/j.bpj.2019.01.013. Epub 2019 Jan 22.

An internally quenched peptide as a new model substrate for rhomboid intramembrane proteases.
Arutyunova E, Jiang Z, Yang J, Kulepa AN, Young HS, Verhelst S, O'Donoghue AJ, Lemieux MJ.
Biol Chem. 2018 Nov 27;399(12):1389-1397. doi: 10.1515/hsz-2018-0255.

Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus.
Panigrahi R, Matsui T, Song AH, Caldo KMP, Young HS, Weselake RJ, Lemieux MJ.
Sci Rep. 2018 Nov 12;8(1):16665. doi: 10.1038/s41598-018-34339-1.

Skin cells prefer a slower calcium pump.
Robia SL, Young HS.
J Biol Chem. 2018 Mar 16;293(11):3890-3891. doi: 10.1074/jbc.H118.002088.

The SarcoEndoplasmic Reticulum Calcium ATPase.
Primeau JO, Armanious GP, Fisher ME, Young HS.
Subcell Biochem. 2018;87:229-258. doi: 10.1007/978-981-10-7757-9_8. Review.

Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA.
Smeazzetto S, Armanious GP, Moncelli MR, Bak JJ, Lemieux MJ, Young HS, Tadini-Buoninsegni F.
J Biol Chem. 2017 Dec 29;292(52):21330-21339. doi: 10.1074/jbc.M117.794453. Epub 2017 Oct 29.

Structure-Function Relationship of the SERCA Pump and Its Regulation by Phospholamban and Sarcolipin.
Gorski PA, Ceholski DK, Young HS.
Adv Exp Med Biol. 2017;981:77-119. doi: 10.1007/978-3-319-55858-5_5. Review.

The Structure of Mammalian Prions and Their Aggregates.
Vázquez-Fernández E, Young HS, Requena JR, Wille H.
Int Rev Cell Mol Biol. 2017;329:277-301. doi: 10.1016/bs.ircmb.2016.08.013. Epub 2016 Oct 22. Review.

The Structural Architecture of an Infectious Mammalian Prion Using Electron Cryomicroscopy.
Vázquez-Fernández E, Vos MR, Afanasyev P, Cebey L, Sevillano AM, Vidal E, Rosa I, Renault L, Ramos A, Peters PJ, Fernández JJ, van Heel M, Young HS, Requena JR, Wille H.
PLoS Pathog. 2016 Sep 8;12(9):e1005835. doi: 10.1371/journal.ppat.1005835. eCollection 2016 Sep.

Two-Dimensional Crystallization of the Ca(2+)-ATPase for Electron Crystallography.
Glaves JP, Primeau JO, Young HS.
Methods Mol Biol. 2016;1377:421-41. doi: 10.1007/978-1-4939-3179-8_38.

Regulation of the sarcoplasmic reticulum calcium pump by divergent phospholamban isoforms in zebrafish.
Gorski PA, Trieber CA, Ashrafi G, Young HS.
J Biol Chem. 2015 Mar 13;290(11):6777-88. doi: 10.1074/jbc.M114.585604. Epub 2015 Jan 15.

Deception in simplicity: hereditary phospholamban mutations in dilated cardiomyopathy.
Young HS, Ceholski DK, Trieber CA.
Biochem Cell Biol. 2015 Feb;93(1):1-7. doi: 10.1139/bcb-2014-0080. Epub 2014 Dec 1. Review.

Phospholamban C-terminal residues are critical determinants of the structure and function of the calcium ATPase regulatory complex.
Abrol N, Smolin N, Armanious G, Ceholski DK, Trieber CA, Young HS, Robia SL.
J Biol Chem. 2014 Sep 12;289(37):25855-66. doi: 10.1074/jbc.M114.562579. Epub 2014 Jul 29.

Membrane transport piece by piece: production of transmembrane peptides for structural and functional studies.
Kemp G, Fliegel L, Young HS.
Curr Protoc Protein Sci. 2014 Feb 3;75:29.8.1-29.8.28. doi: 10.1002/0471140864.ps2908s75.

Sarco(endo)plasmic reticulum calcium ATPase (SERCA) inhibition by sarcolipin is encoded in its luminal tail.
Gorski PA, Glaves JP, Vangheluwe P, Young HS.
J Biol Chem. 2013 Mar 22;288(12):8456-8467. doi: 10.1074/jbc.M112.446161. Epub 2013 Jan 29.

Mouse prion protein polymorphism Phe-108/Val-189 affects the kinetics of fibril formation and the response to seeding: evidence for a two-step nucleation polymerization mechanism.
Cortez LM, Kumar J, Renault L, Young HS, Sim VL.
J Biol Chem. 2013 Feb 15;288(7):4772-81. doi: 10.1074/jbc.M112.414581. Epub 2013 Jan 2.

Distinct morphological and electrophysiological properties of an elk prion peptide.
Glaves JP, Gorski PA, Alier K, Ma L, Renault L, Primeau JO, Jhamandas JH, Young HS.
Peptides. 2013 Feb;40:49-56. doi: 10.1016/j.peptides.2012.12.011. Epub 2012 Dec 20.

Lethal, hereditary mutants of phospholamban elude phosphorylation by protein kinase A.
Ceholski DK, Trieber CA, Holmes CF, Young HS.
J Biol Chem. 2012 Aug 3;287(32):26596-605. doi: 10.1074/jbc.M112.382713. Epub 2012 Jun 15.

Transmembrane helix 11 is a genuine regulator of the endoplasmic reticulum Ca2+ pump and acts as a functional parallel of β-subunit on α-Na+,K+-ATPase.
Gorski PA, Trieber CA, Larivière E, Schuermans M, Wuytack F, Young HS, Vangheluwe P.
J Biol Chem. 2012 Jun 8;287(24):19876-85. doi: 10.1074/jbc.M111.335620. Epub 2012 Apr 23.

Hydrophobic imbalance in the cytoplasmic domain of phospholamban is a determinant for lethal dilated cardiomyopathy.
Ceholski DK, Trieber CA, Young HS.
J Biol Chem. 2012 May 11;287(20):16521-9. doi: 10.1074/jbc.M112.360859. Epub 2012 Mar 16.

Role of pri-miRNA tertiary structure in miR-17~92 miRNA biogenesis.
Chaulk SG, Thede GL, Kent OA, Xu Z, Gesner EM, Veldhoen RA, Khanna SK, Goping IS, MacMillan AM, Mendell JT, Young HS, Fahlman RP, Glover JN.
RNA Biol. 2011 Nov-Dec;8(6):1105-14. doi: 10.4161/rna.8.6.17410. Epub 2011 Nov 1.

Phosphorylation and mutation of phospholamban alter physical interactions with the sarcoplasmic reticulum calcium pump.
Glaves JP, Trieber CA, Ceholski DK, Stokes DL, Young HS.
J Mol Biol. 2011 Jan 21;405(3):707-23. doi: 10.1016/j.jmb.2010.11.014. Epub 2010 Nov 23.

Helical crystallization of two example membrane proteins MsbA and the Ca(2+)-ATPase.
Glaves JP, Fisher L, Ward A, Young HS.
Methods Enzymol. 2010;483:143-59. doi: 10.1016/S0076-6879(10)83007-1.

Effects of phospholamban transmembrane mutants on the calcium affinity, maximal activity, and cooperativity of the sarcoplasmic reticulum calcium pump.
Trieber CA, Afara M, Young HS.
Biochemistry. 2009 Oct 6;48(39):9287-96. doi: 10.1021/bi900852m.

Akt increases sarcoplasmic reticulum Ca2+ cycling by direct phosphorylation of phospholamban at Thr17.
Catalucci D, Latronico MVG, Ceci M, Rusconi F, Young HS, Gallo P, Santonastasi M, Bellacosa A, Brown JH, Condorelli G.
J Biol Chem. 2009 Oct 9;284(41):28180-28187. doi: 10.1074/jbc.M109.036566. Epub 2009 Aug 19.

Cyclopiazonic acid is complexed to a divalent metal ion when bound to the sarcoplasmic reticulum Ca2+-ATPase.
Laursen M, Bublitz M, Moncoq K, Olesen C, Møller JV, Young HS, Nissen P, Morth JP.
J Biol Chem. 2009 May 15;284(20):13513-13518. doi: 10.1074/jbc.C900031200. Epub 2009 Mar 16.

Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy.
Ogawa H, Qiu Y, Huang L, Tam-Chang SW, Young HS, Misono KS.
FEBS J. 2009 Mar;276(5):1347-55. doi: 10.1111/j.1742-4658.2009.06870.x.

Peptide inhibitors use two related mechanisms to alter the apparent calcium affinity of the sarcoplasmic reticulum calcium pump.
Afara MR, Trieber CA, Ceholski DK, Young HS.
Biochemistry. 2008 Sep 9;47(36):9522-30. doi: 10.1021/bi800880q. Epub 2008 Aug 15.

Structural characterization of Ca(2+)-ATPase-bound phospholamban in lipid bilayers by solid-state nuclear magnetic resonance (NMR) spectroscopy.
Seidel K, Andronesi OC, Krebs J, Griesinger C, Young HS, Becker S, Baldus M.
Biochemistry. 2008 Apr 15;47(15):4369-76. doi: 10.1021/bi7024194. Epub 2008 Mar 21.

Dimeric structure of human Na+/H+ exchanger isoform 1 overproduced in Saccharomyces cerevisiae.
Moncoq K, Kemp G, Li X, Fliegel L, Young HS.
J Biol Chem. 2008 Feb 15;283(7):4145-54. doi: 10.1074/jbc.M704844200. Epub 2007 Dec 12.

The molecular basis for cyclopiazonic acid inhibition of the sarcoplasmic reticulum calcium pump.
Moncoq K, Trieber CA, Young HS.
J Biol Chem. 2007 Mar 30;282(13):9748-9757. doi: 10.1074/jbc.M611653200. Epub 2007 Jan 26.

Rational design of peptide inhibitors of the sarcoplasmic reticulum calcium pump.
Afara MR, Trieber CA, Glaves JP, Young HS.
Biochemistry. 2006 Jul 18;45(28):8617-27. doi: 10.1021/bi0523761.

Interactions between Ca2+-ATPase and the pentameric form of phospholamban in two-dimensional co-crystals.
Stokes DL, Pomfret AJ, Rice WJ, Glaves JP, Young HS.
Biophys J. 2006 Jun 1;90(11):4213-23. doi: 10.1529/biophysj.105.079640. Epub 2006 Mar 13.

Determination of membrane protein structure and dynamics by magic-angle-spinning solid-state NMR spectroscopy.
Andronesi OC, Becker S, Seidel K, Heise H, Young HS, Baldus M.
J Am Chem Soc. 2005 Sep 21;127(37):12965-74. doi: 10.1021/ja0530164.

The effects of mutation on the regulatory properties of phospholamban in co-reconstituted membranes.
Trieber CA, Douglas JL, Afara M, Young HS.
Biochemistry. 2005 Mar 8;44(9):3289-97. doi: 10.1021/bi047878d.

Rapid, high-yield expression and purification of Ca2+-ATPase regulatory proteins for high-resolution structural studies.
Douglas JL, Trieber CA, Afara M, Young HS.
Protein Expr Purif. 2005 Mar;40(1):118-25. doi: 10.1016/j.pep.2004.11.015.

The mechanics of calcium transport.
Young HS, Stokes DL.
J Membr Biol. 2004 Mar 15;198(2):55-63. doi: 10.1007/s00232-004-0666-y. Review.

Alpha-bungarotoxin binding to acetylcholine receptor membranes studied by low angle X-ray diffraction.
Young HS, Herbette LG, Skita V.
Biophys J. 2003 Aug;85(2):943-53. doi: 10.1016/S0006-3495(03)74533-0.

Variable symmetry in Salmonella typhimurium flagellar motors.
Young HS, Dang H, Lai Y, DeRosier DJ, Khan S.
Biophys J. 2003 Jan;84(1):571-7. doi: 10.1016/S0006-3495(03)74877-2.

Locating phospholamban in co-crystals with Ca(2+)-ATPase by cryoelectron microscopy.
Young HS, Jones LR, Stokes DL.
Biophys J. 2001 Aug;81(2):884-94. doi: 10.1016/S0006-3495(01)75748-7.

Structure of Na+,K+-ATPase at 11-A resolution: comparison with Ca2+-ATPase in E1 and E2 states.
Rice WJ, Young HS, Martin DW, Sachs JR, Stokes DL.
Biophys J. 2001 May;80(5):2187-97. doi: 10.1016/S0006-3495(01)76191-7.

Locating the thapsigargin-binding site on Ca(2+)-ATPase by cryoelectron microscopy.
Young HS, Xu C, Zhang P, Stokes DL.
J Mol Biol. 2001 Apr 27;308(2):231-40. doi: 10.1006/jmbi.2001.4558.

Three-dimensional crystals of Ca2+-ATPase from sarcoplasmic reticulum: merging electron diffraction tilt series and imaging the (h, k, 0) projection.
Shi D, Lewis MR, Young HS, Stokes DL.
J Mol Biol. 1998 Dec 18;284(5):1547-64. doi: 10.1006/jmbi.1998.2283.

Co-reconstitution and co-crystallization of phospholamban and Ca(2+)-ATPase.
Young HS, Reddy LG, Jones LR, Stokes DL.
Ann N Y Acad Sci. 1998 Sep 16;853:103-15. doi: 10.1111/j.1749-6632.1998.tb08260.x.

How to make tubular crystals by reconstitution of detergent-solubilized Ca2(+)-ATPase.
Young HS, Rigaud JL, Lacapère JJ, Reddy LG, Stokes DL.
Biophys J. 1997 Jun;72(6):2545-58. doi: 10.1016/S0006-3495(97)78898-2.

Lamellar stacking in three-dimensional crystals of Ca(2+)-ATPase from sarcoplasmic reticulum.
Cheong GW, Young HS, Ogawa H, Toyoshima C, Stokes DL.
Biophys J. 1996 Apr;70(4):1689-99. doi: 10.1016/S0006-3495(96)79731-X.

Bilayer structure and physical dynamics of the cytochrome b5 dimyristoylphosphatidylcholine interaction.
Chester DW, Skita V, Young HS, Mavromoustakos T, Strittmatter P.
Biophys J. 1992 May;61(5):1224-43. doi: 10.1016/S0006-3495(92)81932-X.

Molecular basis for the inhibition of 1,4-dihydropyridine calcium channel drugs binding to their receptors by a nonspecific site interaction mechanism.
Young HS, Skita V, Mason RP, Herbette LG.
Biophys J. 1992 May;61(5):1244-55. doi: 10.1016/S0006-3495(92)81933-1.

Analysis of tissue free fatty acids isolated by aminopropyl bonded-phase columns.
Prasad MR, Jones RM, Young HS, Kaplinsky LB, Das DK.
J Chromatogr. 1988 Jul 15;428(2):221-8. doi: 10.1016/s0378-4347(00)83912-2.